Bertholletia excelsa SEEDS IN THE CERRADO-AMAZON TRANSITION REGION: MORPHOMETRY, COLORIMETRY, VIABILITY AND GERMINATION
DOI:
10.31413/nat.v11i2.15580Keywords:
Amazon rainforest; forest residue; recalcitrance; Brazil nuts conservation; extractivism.Abstract
This study aimed to describe the morphometric characteristics of fruits and seeds, and to evaluate the viability and germination of B. excelsa seeds stored under litter under microenvironmental conditions of their natural habitat in the Cerrado-Brazilian Amazon transition. The morphometric characterization included measurements of diameters and latitudinal and longitudinal circumferences of the fruits; exocarp and mesocarp thickness, weight of fruits and seeds per fruit, number of seeds per fruit, thickness, width and length of seeds. Seed moisture was obtained by the ratio between masses. The colorimetric characterization occurred in the mesocarp, external and internal tegument and in the internal part of the seed. Seed viability was evaluated by the tetrazolium test and germination with degummed and intact seeds. Morphometric variability of fruits and seeds of B. excelsa was observed regardless of humidity. The color of the fruit and the outer seed coat changed color with the reduction of relative air humidity. Variations in the relative humidity of the air during the storage period influenced the significant reduction of 45.31% in the water content of the seeds and compromised the physiological quality, reducing the viability and germination capacity of the seeds. The seeds of B. excelsa are sensitive to variations in humidity. The storage of seeds inside the fruit under the litter under microenvironmental conditions of their natural habitat for up to 96 days with average relative humidity above 65%, guarantees the maintenance of seed moisture above the critical humidity of 30%. Viable seeds with germination power have a water content above 45%.
References
ABBADE, L. C.; TAKAKI, M. Teste de tetrazólio para avaliação da qualidade de sementes de Tabebuia roseoalba (Ridl.) Sandwith - Bignoniaceae, submetidas ao armazenamento. Revista Árvore, v. 38, n. 2, p. 233-240, 2014. https://dx.doi.org/10.1590/S0100- 67622014000200003
ALVES, M. A. B.; BORELLA, D. R.; LUZ, C. C. S.; CASTAGNA, D.; SILVA, W. C.; SILVA, A. F.; ALMEIDA, F. T.; SOUZA, A. P. Soil classes in the Caiabi and Renato river basins, tributaries of the Teles Pires river in the south of the Amazon. Nativa, v. 10, n. 3, p. 431-448, 2022. https://doi.org/10.31413/nativa.v10i3.14192
ANDRADE, V. L.; FLORES, B. M.; LEVIS, C.; CLEMENT, C. R.; ROBERTS, P.; SCHONGART, J. Growth rings of Brazil nut trees (Bertholletia excelsa) as a living record of historical human disturbance in Central Amazonia. Plos One, v. 14, e0214128, 2019. https://doi.org/10.1371/journal.pone.0214128
BALDONI, A.B.; TEODORO, L. P. R.; TEODORO, P. R.; HÉLIO TONINI, H.; TARDIN, F. D.; BOTIN, A. A.; EULÁLIA SOLER SOBREIRA HOOGERHEIDE, S.; BOTELHO, S. C. C.; LULU, J.; NETO, A. L. F.; AZEVEDO, V. C. R. Genetic diversity of Brazil nut tree (Bertholletia excelsa Bonpl.) in Southern Brazilian Amazon. Forest Ecology and Management, v. 458, 117795, 2020. https://doi.org/10.1016/j.foreco.2019.117795
BARBEDO, C. J. A new approach towards the so-called recalcitrant seeds. Journal of Seed Science, v. 40, n. 3, p. 221-236, 2018. http://dx.doi.org/10.1590/2317-1545v40n3207201
BORELLA, D. R.; SILVA, A. C.; SOUZA, A. P.; BOUVIE, L.; N OGUEIRA LAS, PEREIRA CAL, SILVA MALV. Biometry of fruit and seeds of brazil chestnut originiting of the Medium-North region of Mato Grosso state. Nativa, v. 5, n. esp, p. 628-633, 2017. http://dx.doi.org/10.5935/2318-7670.v05nespa01
BORELLA, D. R.; SILVA, A. C.; SOUZA, J. H. G.; MARTIM, C. C.; PIZZATTO, M.; SILVA, K. N. C. Viability of brazil nuts seeds by tetrazolium test. Nativa, v. 8, n. 2, p. 336-343, 2020. http://dx.doi.org/10.31413/nativa.v8i3.9333
BORGES, F. A.; TONINI, H.; BALDONI, A. B.; BOTELHO, S. C. C. Size of sample to estimate native seed production of the Brazil nuts. Nativa, v. 4, n. 1, p. 166-169, 2016. http://dx.doi.org/10.14583/2318-7670.v04n03a09
BOUVIE, L.; BORELLA, D. R.; PORTO, P. A. O.; SILVA, A. C.; LEONEL, S. Physico-chemical characterization of fruit’s castanheira of Brazil. Nativa, v. 4, n. 1, p. 107-111, 2016. http://dx.doi.org/10.14583/2318-7670.v04n02a10
BURTIN, P.; JAY-ALLEMAND, C.; CHARPENTIER, J. P.; JANIN, G. Natural wood colouring process in Juglans sp. (J. nigra, J. regia and hybrid J. nigra 23 x J. regia) depends on native phenolic compounds accumulated in the transition zone between sapwood and heartwood. Trees, v. 12, n. 5, p. 258-264, 1998.
BRASIL_Ministério da Agricultura e Reforma Agrária. Regras para análise de sementes. Brasília: MAPA/ACS, 2009. 399p.
CAMARGO, I. P.; CARVALHO, M. M.; VIEIRA, M. G. G. C. Avaliação da deterioração em sementes de castanheira-do-Brasil pelo teste de tetrazólio. Pesquisa Agropecuária Brasileira, v. 32, p. 835-839, 1997.
CAMARGO, I. P.; CASTRO, E. M.; GAVILANES, M. L. Aspectos da anatomia e morfologia de amêndoas e plântulas de Castanheira-do-Brasil. Cerne, v. 6, n. 1, p. 11-18, 2000.
CAMARGOS, J. A. A.; GONÇALEZ, J. C. A colorimetria aplicada como instrumento na elaboração de uma tabela de cores de madeira. Brasil Florestal, v. 20, p. 30-41, 2001.
CAMARGO, F. F.; COSTA, R. B.; RESENDE, M. D. V.; ROA, R. A. R.; RODRIGUES, N. B.; SANTOS, L. V.; FREITAS, A. C. A. Genetic variability for morphometric characteristics in brazilian nut parent trees from northern Mato Grosso, Amazon rain forest. Acta Amazonica, v. 40, p. 705-710, 2010. https://doi.org/10.1590/S0044-59672010000400010
CARPEJANI, A. A.; PENA, G. F.; VIEIRA, F. S.; TIAGO, P. V.; ROSSI, A. A. B. Genetic diversity of Bertholletia excelsa Bonpl.: a native species of the Amazon Rainforest. Floresta e Ambiente, v. 27, e20180180, 2020. https://doi.org/10.1590/2179-8087-FLORAM-2018-0180
COELHO, L. M.; LOPES, M. T. G.; CHAGAS, E. A.; PIO, R.; PASQUAL, M.; COSTA NETO, P. Q.; MAGALHÃES, M. A. Genetic diversity in populations of Brazil nut. Crop Breeding and Applied Biotechnology, v. 17, p. 382-389, 2017. http://dx.doi.org/10.1590/1984-70332017v17n4a57
FRANÇA-NETO, J. B.; KRZYZANOWSKI, F. C. Tetrazolium: an important test for physiological seed quality evaluation. Journal of Seed Science, v. 41, p. 359-366, 2019. http://dx.doi.org/10.1590/2317-1545v41n3223104
IBGE_INSTITUTO BRASILEIRO DE GEOGRAFIA E ESTATÍSTICA. Produção da extração vegetal e da silvicultura. Rio de Janeiro: IBGE, v.36, 8p. 2021. https://biblioteca.ibge.gov.br/visualizacao/periodicos/74/pevs_2021_v36_informativo.pdf
KAINER, K. A.; WADT, L. H. O.; STAUDHAMMER, C. L. Explaining variation in Brazil nut fruit production. Forest Ecology and Management, v. 250, p. 244-255, 2007. https://doi.org/10.1016/j.foreco.2007.05.024
LIMA, D. S.; EGEA, M. B.; CABASSA, I. C. C.; ALMEIDA, A. B.; SOUSA, T. L.; LIMA, T. M.; LOSS, R. A.; VOLP, A. C. P.; VASCONCELOS, L. G.; DALL’OGLIO, E. L.; HERNANDES, T.; TAKEUCHI, K. P. Technological quality and sensory acceptability of nutritive bars produced with Brazil nut and baru almond coproducts, LWT - Food Science and Technology, v. 137, 110467, 2021. https://doi.org/10.1016/j.lwt.2020.110467
MMA_Ministério do Meio Ambiente, Brasil. Portaria MMA n° 443, of December 18, 2014. Disponível em: https://www.ibama.gov.br/component/legislacao/?view=legislacao&legislacao=134519
MÜLLER, C. H.; RODRIGUES, I. A.; MÜLLER, A. A.; MÜLLER, N. R. M. Castanha-do-Brasil: resultados de pesquisa. Belém: EMBRAPA – CPATU, 1980. 25 p
PAMMENTER, N. W.; BERJAK, P. Physiology of desiccation-sensitive (recalcitrant) seeds and the implications for cryopreservation. International Journal of Plant Sciences, v. 175, p. 21-28, 2014. https://dx.doi.org/10.1086/673302
PERES, C. A.; BAIDER, C. Seed dispersal, spatial dritribution and population structure of Brazil nut tree (Bertholletia excelsa) in southeastern Amazonia. Journal of Tropical Ecology, Cambridge, v. 13, n. 4, p. 595-616, 1997. DOI: https://doi.org/10.1017/S0266467400010749
ROCKWELL, C. A.; GUARIGUATA, M. R.; MENTON, M.; QUISPE, E. A.; QUAEDVLIEG, J.; WARREN-THOMAS, E.; SILVA, H. F.; ROJAS, E. E. J.; ARRUNÁTEGUI, J. A. H. K.; VEGA, L. A. M.; VERA, O. R.; HANCCO, R. Q.; TITO, J. F. V.; PANDURO, B. T. V.; SALAS, J. J. Y. Nut production in Bertholletia excelsa across a logged forest mosaic: implications for multiple forest use. Plos One, v. 10, e0135464, 2015. https://doi.org/10.1371/journal.pone.0135464
SALOMÃO, R. P. Densidade, estrutura e distribuição espacial de Castanheira-do-Brasil (Bertholletia excelsa H. & B.) em dois platôs de floresta ombrófila densa na Amazônia setentrional brasileira. Boletim do Museu Paraense Emílio Goeldi, v. 4, n. 1, p. 11-25, 2009.
SANTOS, J. U. M.; BASTOS, M. N. C.; GURGEL, E. S. C.; CARVALHO, A. N. M. Bertholletia excelsa Humboldt & Bonpland (Lecythidaceae): morphological aspects of fruit, seed and seedling. Boletim do Museu Paraense Emílio Goeldi, v. 1, p. 103-112, 2006.
SCHONGART, J.; GRIBEL, R.; FONSECA-JUNIOR, S. F.; HAUGAASEN. T. Age and Growth Patterns of Brazil Nut Trees (Bertholletia excelsa Bonpl.) in Amazonia, Brazil. Biotropica, v. 47, n. 5, p. 550-558, 2015. https://doi.org/10.1111/btp.12243
SCOLES, R.; GRIBEL, R.; KLEIN, N. G. Crescimento e sobrevivência de castanheira (Bertholletia excelsa Bonpl.) em diferentes condições ambientais na região do rio Trombetas, Oriximiná, Pará. Boletim do Museu Paraense Emílio Goeldi, Belém, v. 6, n. 3, p. 273-293, 2011.
SILVA, R. A. F.; SETTER, C.; MAZETTE, S. S.; MELO, R. R.; STANGERLIN, D. M. Colorimetry of wood from thirty tropical species. Brazilian Journal of Wood Science, v. 8, p. 36-41, 2017. http://dx.doi.org/10.12953/2177-6830/rcm.v8n1p36-41
SOUZA, C. D. R.; SILVA, K. C. Energy potential of waste from Brazil nut (Bertholletia excelsa H.B.K.) for production of activated carbon. Research, Society and Development, v. 10, e53310212698, 2021. http://dx.doi.org/10.33448/rsd-v10i2.12698
SOUZA, A. P.; MOTA, L. L.; ZAMADEI, T.; MARTIM, C. C.; ALMEIDA, F. T.; PAULINO, J. Climate Classification and climatic water balance in Mato Grosso state, Brazil. Nativa, v. 1, n. 1, p. 34-43, 2013. http://dx.doi.org/10.14583/2318-7670.v01n01a07
SONEGO, M.; FLECK, C.; PESSAN, L. A. Mesocarp of Brazil Nut (Bertholletia excelsa) as inspiration for new impact resistant materials. Bioinspiration & Biomimetics, v. 14, e056002, 2019. https://doi.org/10.1088/1748-3190/ab2298
SONEGO, M.; MADIA, M.; EDER, M.; FLECK, C.; PESSAN, L. A. Microstructural features influencing the mechanical performance of the Brazil nut (Bertholletia excelsa) mesocarp. Journal of the Mechanical Behavior of Biomedical Materials, v. 116, e104306, 2021. https://doi.org/10.1016/j.jmbbm.2020.104306
SPERA, S. T.; MAGALHÃES, C. A. S.; BALDONI, A. B.; CALDERANO, S. B. Pedological characterization at places of occurrence of natural populations of Brazil nuts in the State of Mato Grosso. Nativa, v. 7, n. 1, p. 145-161, 2019. http://dx.doi.org/10.31413/nativa.v7i2.6502
TONINI, H.; BALDONI, A. B. Estrutura e regeneração de Bertholletia excelsa Bonpl. em castanhais nativos da Amazônia. Ciência Florestal, v. 29, n. 2, p. 607-621, 2019. https://doi.org/10.5902/1980509822112
TONINI, H.; PEDROZO, C. A. Changes in annual production of fruits and seeds of Brazilian nuts trees (Bertholletia excelsa Bonpl., Lecythidaceae) in native forests of Roraima state, Brazil. Revista Árvore, v. 38, p. 133-144, 2014. http://dx.doi.org/10.1590/S0100-67622014000100013
WADT, L. H. O.; FAUSTINO, C. L.; STAUDHAMMER, C. L.; KAINER, K. A.; EVANGELISTA, J. S. Primary and secondary dispersal of Bertholletia excelsa: implications for sustainable harvests. Forest Ecology and Management, v. 415-416, p. 98-105, 2018. https://doi.org/10.1016/j.foreco.2018.02.014
VIEIRA, S.; TRUMBORE, S.; CAMARGO, P. B.; SELHORST, D.; CHAMBERS, J. Q.; HIGUCHI, N.; MARTINELLI, L. A. Slow growth rates of Amazonian trees: Consequences for carbon cycling. Proceedings of the National Academy of Sciences of United States of America, v. 102, n. 51, 18502–18507, 2005.
ZUIDEMA, P. A. Ecology and management of the Brazil nut tree (Bertholletia excels). Riberalta – Bolívia: PROMAB Series 6, 2003. 111p.
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