STUDY SOME TAXONOMIC ASPECTS OF THE TWO SPECIES Limonium meyeri (Boiss.) Kuntze AND Limonium thouinii (Viv.) Kuntze, IN IRAQ

Shaemaa Muhi Hasson  AL-Amery; Hanan Ahmed Hadi  Al-Qaraawi; Nidaa Adnan  Abu-Serag; Yazi Abdullah  Jassim

doi:10.31413/nat.v12i2.17370

Autores/as

Shaemaa Muhi Hasson AL-Amery shaemaamuhi79@gmail.com
Department of Biology, Faculty of Science, University of Babylon, Al-Hillah, Iraq. https://orcid.org/0009-0005-5118-6516
Hanan Ahmed Hadi Al-Qaraawi sci.hanan.ahmed@uobabylon.edu.iq
Department of Biology, Faculty of Science, University of Babylon , Al-Hillah , Iraq.
Nidaa Adnan Abu-Serag sci.nidaa.adnan@uobabylon.edu.iq
Department of Biology, Faculty of Science, University of Babylon , Al-Hillah , Iraq.
Yazi Abdullah Jassim yaziabdullah2020@gmail.com
College of Science, University of Babylon, Babylon, Iraq. https://orcid.org/0000-0002-4156-6006

DOI:

10.31413/nat.v12i2.17370

Palabras clave:

Plumbaginaceae, pollen grains, anatomy of the stem

Resumen

The research aims to separate two species of the genus Limonium (Limonium meyeri and Limonium tuinii) using some Morphological and anatomical characters as taxonomic evidence. The current research included the study of the micro-characteristics of pollen grains and some anatomical characteristics of the stem and leaf using the light and Scanning electron microscope for separation of the species. The attributes of pollen grains (the size of the pollen, the germinating aperture width, and the surface ornamentation). The average length of the polar axis in the polar view reached 45 µm in L. meyeri and 35 µm in L. thouinii. In addition to the variation in the width of the germination aperture width, which was 17.5 µm in L. meyeri and 7.5 µm in L. thouinii. as well as the variations in the characteristics of the epidermal cells and the cross-section of the stems, are of good taxonomic importance to separate the two species. The research confirmed that the micro-characteristics of pollen grains and the anatomical characteristics are of complementary taxonomic importance to the morphological traits in separating genera and species.

Keywords: Plumbaginaceae; pollen grains; anatomy of the stem.

Aspectos taxonômicos das espécies vegetais Limonium meyeri (Boiss.) Kuntze e Limonium tuinii (Viv.) Kuntze, no Iraque

RESUMO: Esta pesquisa objetivou separar duas espécies do gênero Limonium (Limonium meyeri e Limonium tuinii) utilizando alguns caracteres morfológicos e anatômicos como evidência taxonômica. A presente pesquisa incluiu o estudo das micro características dos grãos de pólen e algumas características anatômicas do caule e da folha utilizando o microscópio óptico e eletrônico de varredura para separação das espécies. Os atributos dos grãos de pólen (tamanho do pólen, largura da abertura de germinação e ornamentação da superfície). O comprimento médio do eixo polar na visão polar atingiu 45 µm em L. meyeri e 35 µm em L. tuinii. Além da variação na largura da abertura de germinação, que foi de 17,5 µm em L. meyeri e 7,5 µm em L. tuinii. As variações nas características das células epidérmicas e na secção transversal dos caules, foram importantes caracteres taxonômicos para separar as duas espécies. A pesquisa confirmou que as micro características dos grãos de pólen e as características anatômicas são de importância taxonômica complementar às características morfológicas na separação de gêneros e espécies.

Palavras-chave: Plumbaginaceae; grãos de pólen; anatomia do caule.

Citas

ANTONELLI-USHIROBIRA, T. M.; BLAINSKI, A.; FERNANDES, H. G.; MOURA-COSTA, G. F.; COSTA, M. A.; CAMPOS-SHIMADA, L. B.; SALGUEIRO-PAGADIGORRIA, C. L.; KANESHIMA, E. N.; BECKER, T. C. A.; LEITE - MELLO, E. V. S. and MELLO, J. C. P. Acute toxicity and long-safety evaluation of the crude extract from rhizomes of Limonium brasiliense in mice and rats. Journal of Ethnopharmacology, v. 174, p. 293-298, 2015. https://doi.org/10.1016/j.jep.2015.08.022

AYTUG, B.; AYRUT, S.; MEREV, N.; EDIS, G. Atlas des pollens des environs d’Istanbul. Kutulmus Matbaasi. Istanbul, 1971. 330p.

BAYSAL, I.; EKIZOGLU, M.; ERTAS, A.; TEMIZ, B.; AGALAR, H. G.; CIFTCI, S. Y., TEMEL, H.; GULBERK UCAR, G.; TURKMENOGLU, F. P. Identification of phenolic compounds by LC-MS/MS and evaluation of bioactive properties of two edible halophytes: Limonium effusum and L. sinuatum. Molecules, v. 26, n. 13, e4040, 2021. https://doi.org/10.3390/molecules26134040

BENMEDDOUR, T.; LAOUER, H.; FLAMINI, G.; SALAH, A. Chemical composition of essential oil of Limonium bonduellei. Chemistry of Natural Compounds, v. 54, p. 188-190, 2018. https://doi.org/10.1007/s10600-018-2293-3

CAPERTA, A. D.; ROIS, A. S.; TEIXEIRA, G.; GARCIA-CAPRROS, P.; FLOWERS, T. J. Secretory structures in plants: lessons from the Plumbaginaceae on their origin, evolution, and roles in stress Tolerance. Plant, Cell & Environment, v. 43, n. 12, p. 2912-2931, 2020. https://doi.org/10.1111/pce.13825

CHANDA, S.; GHOSH, K. Pollen morphology and its evolutionary significance in Xanthorrhoeaceae. In: FERGUSON, I. K.; MULLER, J. (Eds.). The evolutionary significance of the exine. 1ª ed. London: Academic Press, 1976. p. 527-559.

DARSHETKAR, A. M.; MAURYA, S.; LEE, C.; BAZARRAGCHAA, B.; BATDELGER, G.; JANCHIV, A.; JEONG, E. J.; CHOI, S.; CHOUDHARY, R. K.; KIM, S. Y. Plastome analysis unveils Inverted Repeat (IR) expansion and positive selection in Sea Lavenders (Limonium, Plumbaginaceae, Limonioideae, Limonieae). PhytoKeys, v. 175, p. 89-107, 2021. https://doi.org/10.3897/phytokeys.175.61054

DOḠAN, C.; BAYSAL, E. Ö. Pollen morphology of the coastal species of Turkish Limonium (Plumbaginaceae). Phytotaxa, v. 388, n. 3, p. 212-228, 2019. https://doi.org/10.11646/phytotaxa.388.3.1

ERDAL, J. A Numerical taxonomic study on the family Plumbaginaceae in Turkey. 119f. Thesis [ Master of Science in Biology] - Middle East Technical University, Ankara - Turkey, 2015. Available on: https://open.metu.edu.tr/handle/11511/25111?show=full

GANCEDO, N. C.; ISOLANI, R.; OLIVEIRA, N. C.; NAKAMURA, C. V.; DANIELA; ARAÚJO, C. M., SANCHES, A. C. C.; TONIN, F. S.; LLIMOS, F. F.; CHIERRITO, D.; MELLO, J. C. P. Chemical constituents, anticancer and anti-proliferative potential of Limonium species: a systematic review. Pharmaceuticals, v. 16, e293, 2023. https://doi.org/10.3390/ph16020293

GANCEDO, N. C.; MEDEIROS, D. C.; GUTIERRE, M. A. M. and MELLOA, J. C. P. Morpho-anatomical characters of Limonium brasiliense leaves. Revista Brasileira de Farmacognosia, v. 28, n. 5, p. 513-519. 2018. https://doi.org/10.1016/j.bjp.2018.05.014

GONZÁLEZ-ORENGA, S.; GRIGORE, M. N.; BOSCAIU, M. ; VICENTE, O. Review constitutive and induced salt tolerance mechanisms and potential uses of Limonium Mill. species. Agronomy, v. 11, e413, 2021. https://doi.org/10.3390/agronomy11030413

HALBRITTER, H.; ULRICH, S.; GRRĺMSSON, F.; WEBER, M.; ZETTER, R.; HESSE, M.; BUCHNER, R.; SVOJTKA, M.; FROSCH-RADIVO, A. Methods in Palynology. In: Illustrated Pollen Terminology. Springer, Cham, 2018. p. 97-127. https://doi.org/10.1007/978-3-319-71365-6_6

KOUTROUMPA, K.; THEODORIDIES, S.; WARREN, B. H.; JIMENEZ, A.; CELEP, F.; DOĞAN, M.; ROMEIRAS, M. M.; SANTOS-GUERRA, A.; FERNÁNDEZ-PALACIOS, J. M.; CASTELLS, J. C.; MOURA, M.; SEQUEIRA, M. M.; CONTI, E. An expanded molecular phylogeny of Plumbaginaceae, with emphasis on Limonium (sea lavenders): taxonomic implications and biogeographic considerations. Ecology and Evolution, v. 8, n. 24, p. 12283-13050, 2019. https://doi.org/10.1002/ece3.4553

LEFAHAL, M.; MAKHLOUFI , E. L. H.; ZAABAT, N.; MEDJROUBI, K.; MERZOUG, B.; LAOUER, H.; AKKAL, S. In vitro antioxidant and photoprotective activities of ethyl acetate fraction of hydroalcoholic extract of the aerial parts of Algerian Plumbaginaceae Limonium thouinii (viv.) Kuntze. In: INTERNATIONAL IVEK BIO CONGRESS. Proceedings... Istanbul – Turkey, 2018. 2p. Available on: https://www.researchgate.net/publication/330025995

SINGH, K.; NAIDOO, Y.; BAIJNATH, H. A Comprehensive review on the genus Plumbago with focus on Plumbago auriculata (Plumbaginaceae). African Journal of Traditional, Complementary and Alternative Medicines, v. 15, n. 1, p. 199-215, 2018. https://doi.org/10.21010/ajtcam.v15i1.21

TALEBI, S. M.; NOORI, M.; NANIZ, H. A. A study of epidermal leaf anatomy of 18 Euphorbia taxa from Kerman Province, Iran. Biologija v. 63, n. 2, p. 126-133, 2017. https://doi.org/10.6001/biologija.v63i2.3524

WALKER, J. W.; DOYLE, J. A. The Bases of Angiosperm Phylogeny: Palynology. Annals of the Missouri Botanical Garden, v. 62, n. 3, p. 664-723, 1975.

YURCHYSHYN , O. I.; RUSKO, G. V.; KUTSYK , R.V. Antimicrobial effect of south Ukrainian sea lavender Limonium meyeri (Boiss.) O. Kuntze and Limonium hypanicum Klok. with especial emphasizing against staphylococci and Propionibacteria. The Pharma Innovation Journal, v. 6, n. 10, p. 380-384, 2017.