EVALUATION OF CHEMICAL COMPOSITION AND ANTIBACTERIAL ACTIVITY OF METHANOLIC EXTRACTS FROM NINE MEDICINAL PLANTS AGAINST STANDARD BACTERIAL STRAINS IN VITRO

Authors

  • Tayseer Ali Talab taranoom.isi.isc@gmail.com
    Department of Pharmacology, College of Medicine, University of Thi-Qar, Nasiriyah, Iraq. https://orcid.org/0000-0003-2069-8840
  • Abolfazl Jafari Sales a.jafari_1392@yahoo.com
    Department of Microbiology, Kaz.C., Islamic Azad University, Kazerun, Iran. https://orcid.org/0000-0002-5710-4076
  • Safa Meskini-Marandi safamarandi07@gmail.com
    Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran. / Department of Cellular and Molecular Biology, Ta.C., Islamic Azad University, Tabriz, Iran. https://orcid.org/0009-0003-3670-0121
  • Shabnam Khakpour-Ziaei sh.khakpourr@gmail.com
    Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran. / Department of Cellular and Molecular Biology, Ta.C., Islamic Azad University, Tabriz, Iran. https://orcid.org/0009-0002-1462-4079
  • Mehrdad Pashazadeh mehrdadpashazadeh85@gmail.com
    Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran. / Department of Medical Laboratory Sciences and Microbiology, TaMS.C., Islamic Azad University, Tabriz, Iran. https://orcid.org/0009-0005-0694-4592
  • Ali Esmail Al-Snafi aboahmad61@yahoo.com
    Department of Pharmacology, College of Medicine, University of Thi-Qar, Nasiriyah, Iraq. https://orcid.org/0000-0002-0239-028X
  • Mahdi Murshd Thuwaini taranoom.isii.iscc@gmail.com
    College of Medical and Health Techniques, Southern Technical University- Basrah, Iraq. https://orcid.org/0000-0002-7607-3076

DOI:

https://doi.org/10.31413/nat.v13i3.19581


Keywords:

Plants;, Medicinal plants;, Antibacterial activity;, Pathogenic bacteria;, Gram-positive bacteria; , Gram-negative bacteria;

Abstract

This research aims to identify the antibacterial properties of medicinal plants, which are crucial in traditional medicine due to their natural origin and high safety. Nine medicinal plants from East Azerbaijan Province were gathered for experimental-laboratory investigation, with essential oils extracted and gas chromatography-mass spectrometry analysis used to identify active components. Additionally, methanolic extracts were prepared at different concentrations using the Soxhlet method, and their effects on bacteria were evaluated through the agar well diffusion method, as well as MIC and MBC tests. At 400 mg mL-1, all plant extracts in this investigation exhibited the strongest antibacterial activity. When it came to Gram-positive bacteria like Staphylococcus aureus and Bacillus cereus, the antibacterial activity of all plant extracts was noticeably greater than that of Gram-negative bacteria like Pseudomonas aeruginosa and Escherichia coli. The extracts of Glycyrrhiza glabra, Mentha piperita L., Rosa damascena, and Peganum harmala showed the strongest effects against Gram-positive bacteria, according to an evaluation of MIC and MBC values. The findings show that all plant methanolic extracts exhibit strong antibacterial properties against Gram-positive bacteria and may be viable therapeutic options. Furthermore, the essential oils of the plants under study included 209 different chemicals.

Keywords: medicinal plants; antibacterial activity; pathogenic bacteria; Gram-positive bacteria; Gram-negative bacteria.

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Published

2025-09-25

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Vol. 13 No. 3 (2025)

Section

Ciências Ambientais / Environmental Sciences

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EVALUATION OF CHEMICAL COMPOSITION AND ANTIBACTERIAL ACTIVITY OF METHANOLIC EXTRACTS FROM NINE MEDICINAL PLANTS AGAINST STANDARD BACTERIAL STRAINS IN VITRO. (2025). Nativa, 13(3), 532-541. https://doi.org/10.31413/nat.v13i3.19581

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