AVALIAÇÃO DE MARCADORES ISSR PARA ESTUDOS MOLECULARES DE BACUPARI
DOI:
https://doi.org/10.31413/nat.v11i3.15205Keywords:
Diversidade Genética, Garcinia brasiliensis, PantanalAbstract
The species Garcinia brasiliensis has various scientifically proven applications in folk medicine, however, the lack of dissemination of these characteristics of the species is a current problem. Among the studies that can enhance this knowledge is the genetic diversity study, for which the use of ISSR molecular markers is recommended for being reliable, rapid, and economical. Therefore, this study aimed to select and classify dominant molecular markers for studies with the G. brasiliensis species. For this, total DNA was extracted from 40 bacupari individuals. Two individuals were selected to perform ISSR primer amplification tests via PCR. 31 primers were tested, which were classified regarding their amplification quality as good, poor, or absent. Out of the 31 tested primers, 13 showed good results regarding the presence and quality of the bands, therefore, they were indicated for use in research aiming the molecular characterization and evaluation of genetic diversity of the species Garcinia brasiliensis.
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