ASEPSIS AND IN VITRO ESTABLISHMENT OF EXPLANTS FROM PINEAPPLE TURIAÇU AMAZONAS CULTIVAR
DOI:
https://doi.org/10.31413/nat.v14i2.20355Keywords:
Ananas comosus, tissue culture, micropropagation, contamination, oxidationAbstract
The objective of this study was to evaluate the use of sodium hypochlorite solution in the asepsis and in vitro establishment of apical and axillary buds extracted from the crown stem of pineapple fruit of the Turiaçu Amazonas cultivar. The experiment was conducted in a completely randomized design, in a factorial scheme, with two types of explants (axillary bud and apical bud), three concentrations of NaClO in the aseptic solution (0.5%, 1.25%, and 2.5% active chlorine), and two explant immersion times in the aseptic solution (5 and 10 minutes). For both bud types, the best results were obtained with 5 minutes of immersion in the aseptic solution, but with a concentration of 0.5% active chlorine for apical bud and 1.25% for axillary bud. The most favorable treatments resulted in the in vitro establishment of 93.8% of axillary buds and 58.3% of apical buds, without contamination or oxidation and suitable for micropropagation. It is concluded that NaClO is effective in the asepsis of in vitro establishment of apical and axillary buds extracted from the fruit crown of the pineapple cultivar Turiaçu Amazonas, and that the appropriate concentration of the aseptic agent depends on the type of explant used.
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