ISOLATION AND IDENTIFICATION NORMAL FLORA BACTERIA FROM DIFFERENT AREAS OF NINAVA GOVERNORATE
DOI:
10.31413/nativa.v11i2.15434Palavras-chave:
isolation, identification, normal flora bacteriaResumo
ABSTRACT: In this study, different types of bacteria were isolated and diagnosed from soils collected from different areas of Ninava governorate, new strain of bacteria was discovered, based on biochemical tests and genetic examination (16S rRNA) and the strain was recorded under accession number (MW690182) in the Gene Bank (NCBI). The isolated bacteria were Bacillus Subtilis strain that given new name (AHM1976) it was positive for gram stain, motility, hemolytic, beta type.
Keywords: isolation; identification; normal flora bacteria.
Isolamento e identificação de bactérias da flora normal de diferentes áreas da província de Ninava, Iraque
RESUMO: Neste estudo, diferentes tipos de bactérias foram isolados e diagnosticados em solos coletados em diferentes áreas da Província de Ninava, Iraque. Foi descoberta uma nova cepa de bactéria, com base em testes bioquímicos e exame genético (16S rRNA), sendo essa cepa registrada sob o número de acesso (MW690182) no Gene Bank (NCBI). A bactéria isolada foi a cepa Bacillus Subtilis, que recebeu novo nome (AHM1976), testando de forma positiva para coloração de gram, motilidade, hemolítica, tipo beta.
Palavras-chave: isolamento; identificação; bactérias da flora normal.
Referências
ABEDIN, J. I. Isolation and identification of cellulose degrading bacteria from soil sample. 80p. Dissertation [B.Sc. in Microbiology]- BRAC University, Dhaka, Bangladesh. 2015. Available on: https://core.ac.uk/download/pdf/61806579.pdf
ABDULLAH, R. M.; MAHDI, A. F. Identification of Pseudomonas aeruginosa from clinical specimen by using 16S rRNA. Journal of Biotechnology Research Center, v. 10, n. 1, p. 45-49, 2016. Available on: https://www.iasj.net/iasj/article/108437
ATLAS, R. M.; CERNIGLIA, C. E. Bioremediation of petroleum pollutants: diversity and environmental aspects of hydrocarbon biodegradation. Bioscience, v. 45, n. 5, p. 332-338, 1995. https://doi.org/10.2307/1312494
AZEEZ, E. M. T.; SHAREEF, A. Y. Ability of Micrococcus Isolation from soil in Biodegradation Diesel. Rafidain Journal of Science, v. 23, n. 8, p. 95-1112, 2012. https://doi.org/10.33899/rjs.2012.64532
CAPPUCCINO, J. G.; SHERMAN, N. Microbiology: a laboratory manual. 6th ed. Menlo Park, CA: Benjamin/Cummings, 2010. 477p.
COLLEE, J. G.; MARMION, B. P.; FRASER, A. G.; SIMMONS, A. (Eds.). Practical Medical Microbiology. 14th Edition, New York: Churchill Livingstone, 1996. 978p.
EARL, A. M.; LOSICK, R.; KOLTER, R. Ecology and genomics of Bacillus subtilis. Trends in Microbiology, v. 16, n. 6, p. 269-275, 2008. https://doi.org/10.1016/j.tim.2008.03.004
FORBESB, B. A.; SAHM, D. F.; WESSIFELD, A. S. (2002). Bailey and Scotts' diagnostic microbiology. 9th Ed. S. Louis Missouri: Mosby Inc., 2002. 509p.
JENKINS, C.; LING, C. L.; CIESIELCZUK, H. L.; LOCKWOOD, J.; HOPKINS, S.; MCHUGH, T. D.; GILLESPIE, S. H.; KIBBLER, C. C. Detection and identification of bacteria in clinical samples by 16S rRNA gene sequencing: comparison of two different approaches in clinical practice. Journal of Medical Microbiology, v. 61, n. 4, p. 483-488, 2012. https://doi.org/10.1099/jmm.0.030387-0
LOGAN, N. A.; DE VOS, P. Genus I. Bacillus Cohn 1872, 174AL. In: De VOS, P.; GARRITY, G. M.; JONES, D.; KRIEG, N. R.; LUDWIG, W.; RAINEY, F. A.; SCHLEIFER, K. H.; WHITMAN, W. B. (Eds.). Bergey’s Manual of Systematic Bacteriology. 2nd Edition, New York: Springer, 2009. v. 3, p. 21-128.
MACFADDIN, J. F. (2013). Biochemical tests for identification of medical bacteria. Philadelphia: Lippincott Williams & Wilkins, 2013. 441p.
MIROUZE, N.; DUBNAU, D. Chance and necessity in Bacillus subtilis development. In: DRIKS, A.; EICHENBERGER, P. (Eds.) The Bacterial Spore: from Molecules to Systems, American Society of Microbiology. Washington: ASM Press, 2016. p. 105-144.
OWAIED, Y. H.; AMEEN, B. M.; JASSIM, M. A. Measuring the viability of Bacillus cereus bacteria on the biodegradation of crude oil in soil incubated at varying temperatures and concentrations. Al-Kufa University Journal for Biology, v. 10, n. 1, p. 118-129, 2018. https://journal.uokufa.edu.iq/index.php/ajb/article/view/8202/7807
SONI, A. K. Isolation and characterization of biosurfactant producing. 34p. Thesis [Bachelor of Technology – Chemical Engineering] – National Institute of Technology Rourkela, Odisha, India, 2013. Available on: http://ethesis.nitrkl.ac.in/5178/1/109CH0493.pdf
WHITMAN, W. B.; COLEMAN, D. C.; WIEBE, W. J. Prokaryotes: the unseen majority. Proceedings of the National Academy of Sciences, v. 95, n. 12, p. 6578-6583, 1998. https://doi.org/10.1073/pnas.95.12.6578
RAMBO, I. M.; MARSH, A.; BIDDLE, J. F. Cytosine methylation within marine sediment microbial communities: potential epigenetic adaptation to the environment. Frontiers in Microbiology, v. 10, e1291, 2019. https://doi.org/10.3389/fmicb.2019.01291
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