EFFICIENT CALLUS INDUCTION FROM VARIOUS EXPLANTS OF Jatropha curcas
DOI:
10.31413/nat.v11i4.16175Palavras-chave:
Jatropha curcas L., callus cultures, cotyledons, Benzyl adenine (BA), naphthalene acetic acid (NAA)Resumo
Jatropha curcas L. is a tropical plant of the Euphorbiaceae family, characterized by its high oil content and ability to grow in various conditions, making it the most promising non-food biofuel crop in the world. The current study was directed to develop effective methods of tissue culture using this plant. The study was able to induce callus from cotyledons of sterile seedlings, as well as from true leaves taken from the field and from the embryo, using MS medium supplemented with interfering concentrations of Naphthalene acetic acid (NAA) with Benzyl adenine (BA), as the results indicated that The medium containing 0.5 mg L-1 for each of them gave the highest percentage of callus induction for the cotyledonous leaves, true leaves and embryonic peduncle, which reached 100%, 80% and 100%, respectively.
Keywords: Jatropha curcas L.; callus cultures; cotyledons; Benzyl adenine (BA); naphthalene acetic acid (NAA).
Indução eficiente de calos a partir de vários explantes de Jatropha curcas
RESUMO: Jatropha curcas L. é uma planta tropical da família Euphorbiaceae, caracterizada por seu alto teor de óleo e sua capacidade de crescer em uma variedade de condições, o que a tornou a cultura de biocombustível não alimentar mais promissora do mundo. O presente estudo foi direcionado para desenvolver métodos eficazes de cultura de tecidos para essa espécie. O estudo foi capaz de induzir calos de cotilédones de mudas estéreis, bem como de folhas verdadeiras retiradas do campo e do embrião, usando meio MS suplementado com concentrações interferentes de ácido naftaleno acético (NAA) com Benzil adenina (BA), como os resultados indicaram que o meio contendo 0,5 mg L-1 para cada um deles proporcionou a maior porcentagem de indução de calos para as folhas cotilédones, folhas verdadeiras e pedúnculo embrionário, que atingiram 100%, 80% e 100%, respectivamente.
Palavras-chave: Jatropha curcas L.; culturas de tecidos; cotilédones; Benzil adenina (BA); ácido naftaleno acético (NAA).
Referências
AKITHA DEVI, M. K.; KUMAR, S. S.; GIRIDHAR, P. High yield production of folates from soybean callus cultures in response to elicitors. 3 Biotech., v. 8, n. 2, p. 1-10, 2018. https://doi.org/10.1007/s13205-018-1101-x
AL-ABASI, I. N.; AL-MALLAH, M. K.; KASAB BASHI, B. Z. Heat Shock Enhancement Salicylic Acid Biosynthesis in Callus of Calendula officinalis L. Journal of Pharmaceutical Sciences and Research, v. 12, n. 7, p. 869-874, 2020.
ALWASH, B. M. J.; SALMAN, Z. O.; HAMAD, S. F. Qualitative and quantitative evaluation of active constituents in callus of Lavandula angustifolia plant in vitro. Baghdad Science Journal, v. 17, n. 2, p. 591-598, 2020. https://orcid.org/0000-0003-4249-2464
CHEN, Y. M.; HUANG, J. Z.; HOU, T. W.; PAN, I. Effects of light intensity and plant growth regulators on callus proliferation and shoot regeneration in the ornamental succulent Haworthia. Botanical Studies, v. 60, n. 1, p. 1-8, 2019. https://doi.org/10.1186/s40529-019-0257-y
COMFORT, A. C.; CYPRIAN, O. U.; AGABA, O. C. E. In vitro plant regeneration from mature embryo explants of Jatropha curcas L. (A Biodiesel Plant) on two standard basal nutrient media. American Journal of Plant Physiology, v. 13, n. 1, p. 23-35, 2018. https://doi.org/10.3923/ajpp.2018.23.35
EL-SAYED, M.; ALY, U. I.; MOHAMED, M. S.; RADY, M. R. In vitro regeneration and molecular characterization of Jatropha curcas plant. Bulletin of the National Research Centre, v. 44, n. 70, p. 1-12, 2020. https://doi.org/10.1186/s42269-020-00320-0
FUFA, H.; SHEKATA, J. D. Development of an in vitro callus induction protocol and shoot proliferation for selected Jatropha (Jatropha curcas) Accessions. Plant Tissue Culture and & Biotechnology, v. 30, n. 1, p. 131-141, 2020. https://doi.org/10.3329/ptcb.v30i1.47798
HEGAZI, G.; EL-HANAFY, N. A.; MOHAMED, A. M. A.; ABU-ELKHEIR, Z. A. In vitro regeneration of the biofuel crop jatropha curcas. Plant Archives, v. 20, n. 2, p. 2122-2127, 2020.
HUTAPEA, A. M.; FADILLAH, Q. Antihyperglycemic and Antioxidant Properties of Jatropha curcas and Jatropha gossypifolia (Linn) Extracts. Journal of Medical Science, v. 16, n. 2, p. 115-122, 2022. https://doi.org/10.26891/JIK.v16i2.2022.115-122
ISLAM, M. R.; BARI, M. A. Immature embryo is the potential source for In vitro plant regeneration in Jatropha curcas. Journal of Bio-Science, v. 20, p. 125-134, 2012. https://doi.org/10.3329/jbs.v20i0.17726
KHEMKLADNGOEN, N.; CARTAGENA, J. A; SHIBAGAKI, N.; FUKUI, K. Adventitious shoot regeneration from juvenile cotyledons of a biodiesel producing plant, Jatropha curcas L. Journal of Bioscience and Bioengineering, v. 111, n. 1, p. 67-70, 2011. https://doi.org/10.1016/j.jbiosc.2010.09.002
KHURANA-KAUL, V.; KACHHWAHA, S.; KOTHARI, S. L. Direct shoot regeneration from leaf explants of Jatropha curcas in response to thidiazuron and high copper contents in the medium. Biologia Plantarum, v. 54, n. 2, p. 369-372, 2010. https://doi.org/10.1007/s10535-010-0066-3
LIU, Y.; TONG, X.; HUI, W.; LIU, T.; CHEN, X.; LI, J.; ZHUANG, C.; YANG, Y.; LIU, Z. Efficient culture protocol for plant regeneration from petiole explants of physiologically mature trees of Jatropha curcas L. Biotechnology & Biotechnological Equipment, v. 29, n. 3, p. 479-488, 2015. https://doi.org/10.1080/13102818.2015.1013308
MOHAMMED, A. A.; YAHYA, R. T. Differentiation of white lupine plants, Lupinus albus L, from the callus of the subcotyledonous stems of their seedling. Journal of Al-Rafidain Sciences, v. 27, n. 4, p. 95-102, 2018.
MURASHIGE, T.; SKOOG, F. A. Revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, v. 15, n. 3, p. 473-497, 1962. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
OSENI, O. A.; AKINDAHUNSI, A. A. Some Phytochemical Properties and Effect of Fermentation on the Seed of Jatropha curcas L. American Journal of Food Technology, v. 6, n. 2, p. 158-165, 2011. https://doi.org/10.3923/ajft.2011.158.165
POMPELLI, M. F.; JARMA-OROZCO, A.; RODRIGUEZ-PAEZ, L. A. Salinity in Jatropha curcas: A Review of physiological, biochemical and molecular factors involved. Agriculture, v. 12, n. 5, e594, 2022. https://doi.org/10.3390/agriculture12050594
RIZWAN, S.; AFTAB, F. Effect of different pretreatments on breaking seed dormancy and In vitro germination in Jatropha curcas L. Pakistan Journal of Botany, v. 50, n. 2, p. 605-608, 2018.
SAPETA, H.; YOKONO, M.; TAKABAYASHI, A.; UENO, Y.; CORDEIRO, A. M.; HARA, T.; TANAKA, A.; AKIMOTO, S.; OLIVEIRA, M. M.; TANAKA, R. Reversible down-regulation of photosystems I and II leads to fast photosynthesis recovery after long-term drought in Jatropha curcas. Journal of Experimental Botany, v. 74, n. 1, p. 336-351, 2023. https://doi.org/10.1093/jxb/erac423
SARABIA, J. B. F.; CALALAS, A. G. S.; GREGORIO, J. P.; MACEREN, J. I. G.; PADAYHAG, W. R. L.; FALLER, E. M.; A review on the medicinal uses and toxicological effects of herbal plant Jatropha curcas l. International Journal of Research Publication and Reviews, v. 3, n. 5, p. 3389-3408, 2022. https://doi.org/10.55248/gengpi.2022.3.5.24
VERMA, Y.; RAWAT, H.; PARVEEN, R.; SAINI, N.; NEGI, N.; MISHRA, A.; GAURAV, N. Potentials and cultivation of bubble bush (Jatropha curcas Linn.) in human welfare: A review, v. 6, n. 3, p. 367-376, 2021. https://www.researchgate.net/publication/351905061
VIJAYALAKSHMI, R.; VETRISELVI, A.; JUNIOR, M. R. E. J.; RODRIGUES, A. P. Chemistry, Biological Activities, and Uses of Jatropha Latex. In: H. N. MURTHY, Gums, Resins and Latexes of Plant Origin. Reference Series in Phytochemistry, p. 825. 2022. https://doi.org/10.1007/978-3-030-76523-1_35-1
ZAHARA, M.; THOMY, Z.; HARNELLY, E. The combination effect of Naphthalene Acetic Acid (NAA) and Benzyl Amino Purine (BAP) on micropropagation of Jatropha curcas L. Jurnal Natural, v. 13, n. 2, p. 23-27, 2013.
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