EFFICIENT CALLUS INDUCTION FROM VARIOUS EXPLANTS OF Jatropha curcas
DOI:
10.31413/nat.v11i4.16175Palavras-chave:
Jatropha curcas L., callus cultures, cotyledons, Benzyl adenine (BA), naphthalene acetic acid (NAA)Resumo
Jatropha curcas L. is a tropical plant of the Euphorbiaceae family, characterized by its high oil content and ability to grow in various conditions, making it the most promising non-food biofuel crop in the world. The current study was directed to develop effective methods of tissue culture using this plant. The study was able to induce callus from cotyledons of sterile seedlings, as well as from true leaves taken from the field and from the embryo, using MS medium supplemented with interfering concentrations of Naphthalene acetic acid (NAA) with Benzyl adenine (BA), as the results indicated that The medium containing 0.5 mg L-1 for each of them gave the highest percentage of callus induction for the cotyledonous leaves, true leaves and embryonic peduncle, which reached 100%, 80% and 100%, respectively.
Keywords: Jatropha curcas L.; callus cultures; cotyledons; Benzyl adenine (BA); naphthalene acetic acid (NAA).
Indução eficiente de calos a partir de vários explantes de Jatropha curcas
RESUMO: Jatropha curcas L. é uma planta tropical da família Euphorbiaceae, caracterizada por seu alto teor de óleo e sua capacidade de crescer em uma variedade de condições, o que a tornou a cultura de biocombustível não alimentar mais promissora do mundo. O presente estudo foi direcionado para desenvolver métodos eficazes de cultura de tecidos para essa espécie. O estudo foi capaz de induzir calos de cotilédones de mudas estéreis, bem como de folhas verdadeiras retiradas do campo e do embrião, usando meio MS suplementado com concentrações interferentes de ácido naftaleno acético (NAA) com Benzil adenina (BA), como os resultados indicaram que o meio contendo 0,5 mg L-1 para cada um deles proporcionou a maior porcentagem de indução de calos para as folhas cotilédones, folhas verdadeiras e pedúnculo embrionário, que atingiram 100%, 80% e 100%, respectivamente.
Palavras-chave: Jatropha curcas L.; culturas de tecidos; cotilédones; Benzil adenina (BA); ácido naftaleno acético (NAA).
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