ÓLEOS ESSENCIAIS DE Blepharocalyx, Nectandra e Piper: ATIVIDADE SOBRE FUNGOS APODRECEDORES DE MADEIRA
DOI:
10.31413/nativa.v10i3.13674Palavras-chave:
biciclogermacreno, deidrofuquinona, dilapiol, eucaliptol, Gloeophyllum trabeum, Trametes versicolorResumo
A busca por produtos naturais que possam substituir preservantes da madeira sintéticos é crescente. Dentre estes produtos, estão os óleos essenciais (OEs). Portanto, o objetivo deste estudo foi avaliar a atividade antifúngica dos OEs de Blepharocalyx salicifolius, Nectandra grandiflora, Nectandra megapotamica e Piper gaudichaudianum, sobre os fungos apodrecedores de madeira Trametes versicolor e Gloeophyllum trabeum. Os OEs foram extraídos por hidrodestilação e foram caracterizados quimicamente. A atividade antifúngica dos OEs foi avaliada pelo teste de difusão em placas de Petri. A verificação da inibição foi calculada com auxílio do software ImageJ®. Os componentes majoritários dos OEs de B. salicifolius e P. gaudichaudianum foram eucaliptol e dilapiol, respectivamente. Biciclogermacreno foi o constituinte majoritário do óleo essencial (OE) de folhas de N. megapotamica, enquanto que a deidrofuquinona foi majoritária nos OEs de folhas e frutos de N. grandiflora. A maior inibição do crescimento de T. versicolor foi causada pelo OE de N. megapotamica, com 31,4%. Os maiores percentuais de inibição observados sobre o crescimento de G. trabeum foram proporcionados pelos extrativos de P. gaudichaudianum e B. salicifolius, com 13,9 e 7,5%, respectivamente. Contudo, o resultado deste último não diferiu dos demais OEs avaliados. Os resultados indicaram que o extrativo de espécie de Nectandra que apresentou maior efeito sobre T. versicolor foi N. megapotamica. Entretanto, este gênero não foi tão eficiente contra G. trabeum.
Palavras-chave: biciclogermacreno; deidrofuquinona; dilapiol; eucaliptol; Gloeophyllum trabeum; Trametes versicolor.
Blepharocalyx, Nectandra and Piper essential oils: activity on wood-decay fungi
ABSTRACT: The search for natural products that can replace synthetic wood preservatives is growing. Among these products are essential oils (EOs). Therefore, the aim of this study was to evaluate the antifungal activity of EOs from Blepharocalyx salicifolius , Nectandra grandiflora , Nectandra megapotamica and Piper gaudichaudianum on the wood-rotting fungi Trametes versicolor and Gloeophyllum trabeum. The EOs were extracted by hydrodistillation and chemically characterized. The antifungal activity of the EOs was evaluated by the Petri dish diffusion test. The inhibition verification was calculated using ImageJ® software. The major components of the EOs of B. salicifolius and P. gaudichaudianum were eucalyptol and dillapiol, respectively. Bicyclogermacrene was the major constituent of N. megapotamica leaves essential oil (EO), while dehydrofuquinone was the major constituent of the EOs from N. grandiflora leaves and fruits. The highest growth inhibition of T. versicolor was caused by N. megapotamica EO, with 31.4%. The highest inhibition percentages of G. trabeum growth were provided by P. gaudichaudianum and B. salicifolius extractives, with 13.9 and 7.5%, respectively. However, the result of the latter did not differ from the other EOs evaluated. The results indicated that, from the extractives of Nectandra species, the greatest effect on T. versicolor was detected to N. megapotamica EO. However, this genus was not so efficient against G. trabeum.
Keywords: bicyclogermacrene; dehydrofuquinone; dillapiole; eucalyptol; Gloeophyllum trabeum; Trametes versicolor.
Referências
ADAMS, R. P. Identification of essential oil components by gas chromatography/quadrupole mass spectroscopy. 4 ed. Illinois: Allured Publishing Corporation, 2009. 804p.
AMARAL, L. P.; SCHINDLER, B.; BIANCHINI, N. H.; LONGHI, S. J.; ALMEIDA, C. A. A. de; MALLMANN, C. A.; HEINZMANN, B. M. Variabilidad de la composición química del aceite esencial de Nectandra megapotamica (Spreng.) Mez (Lauraceae). Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, v. 14, n. 3, p. 190-205, 2015.
BAYRAMOGLU, M.; ARICA, M. Y. Immobilization of laccase onto poly (glycidyl methacrylate) brush grafted poly (hydroxyethyl methacrylate) films: enzymatic oxidation of phenolic compounds. Materials Science and Engineering, v. 6, n. 1, p. 1990–1997, 2009.
BENTO, T. S.; TORRES, L. M. B.; FIALHO, M. B.; BONONI, V. L. R. Growth inhibition and antioxidative response of wood decay fungi exposed to plant extracts of Casearia species. Letters in Applied Microbiology, v. 58, n. 1, p. 79-86, 2014. https://doi.org/10.1111/lam.12159.
BIANCHINI, N. H. Óleo essencial de Nectandra grandiflora Nees & Mart. ex Nees: efeito antifúngico e bioestimulante em cultivos in vitro. 2017. 99p. Dissertação (Mestrado em Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2017.
CAI, L.; JEREMIC, D.; LIM, H.; KIMET, Y. β-Cyclodextrins as sustained-release carriers for natural wood preservatives. Industrial Crops and Products, v. 130, p. 42–48, 2018. https://doi.org/10.1016/j.indcrop.2018.12.061.
CHENG, S.-S.; LIN, C.-Y; GU, H.-J.; CHANG, S.-T. Antifungal activities and chemical composition of wood and leaf essential oils from cunninghamia konishii. Journal of Wood Chemistry and Technology, v. 31, n. 3, p. 204–217, 2011. https://doi.org/10.1080/02773813.2010.515049.
DANIELLI, L. J.; SOUZA, T. J.; MACIEL, A. J.; FERRÃO, M. F.; FUENTEFRIA, A.M.; APEL, M.A. Influence of monoterpenes in biological activities of Nectandra megapotamica (Spreng.) Mez essential oils. Biomolecules, v. 9, n. 3, p. 112, 2019. https://doi.org/10.3390/biom9030112.
FERREIRA, R. G. MONTEIRO, M. C.; SILVA, J. K. R.; MAIA, J. G. S. Antifungal action of the dillapiole-rich oil of Piper aduncum against dermatomycoses caused by filamentous fungi. Journal of Advances in Medicine and Medical Research, v. 15, n. 12, p. 1-10, 2016. 10.9734/BJMMR/2016/26340.
FURTADO, F. B.; BORGES, B. C.; TEIXEIRA, T. L.; GARCES, H. G.; ALMEIDA JUNIOR, L. D. D.; ALVES, F. C. B.; SILVA, C. V.; FERNANDES JUNIOR, A. Chemical composition and bioactivity of essential oil from Blepharocalyx salicifolius. International Journal of Molecular Sciences, v. 19, n. 1, p. 33, 2018. https://doi.org/10.3390/ijms19010033.
HARKAT-MADOURI, L.; ASMA, B.; SAID, Z. B. S.; RIGOU, P.; GRENIER, D.; ALLALOU, H.; REMINI, H.; ADJAOUD, A.; BOULEKBACHE-MAKHLOUF, L. Chemical composition, antibacterial and antioxidant activities of essential oil of Eucalyptus globulus from Algeria. Industrial Crops and Products, v. 78, p. 148–153, 2015. DOI: 10.1016/j.indcrop.2015.10.015.
HOAGLIN, D. C.; IGLEWICZ, B.; TUKEY, J. W. Performance of some resistant rules for outlier labeling. Journal of American Statistical Association, v. 81, p. 991-999, 1986. https://doi.org/10.2307/2289073.
JING, C.; ZHAO, J.; HAN, X.; HUANG, R.; CAI, D.; ZHANG, C. Essential oil of Syringa oblata Lindl. as a potential biocontrol agent against tobacco brown spot caused by Alternaria alternata. Crop Protection, v. 104, p. 41-46, 2018. DOI: 10.1016/j.cropro.2017.10.002.
MEDEIROS, F. C. M.; GOUVEIA, F. N.; BIZZO, H. R.; VIEIRA, R. F.; DEL MENEZZI, C. H. S. Fungicidal activity of essential oils from Brazilian Cerrado species against wood decay fungi. International Biodeterioration & Biodegradation, v. 114, p. 87-93, 2016. http://dx.doi.org/10.1016/j.ibiod.2016.06.003.
MOHAREB, A. S. O.; BADAWY, M. E. I.; ABDELGALEIL, S. A. M. Antifungal activity of essential oils isolated from Egyptian plants against wood decay fungi. Journal of Wood Science, v. 59, p. 499–505, 2013. DOI: 10.1007/s10086-013-1361-3.
MORANDIM, A. D. A.; PIN, A. R.; PIETRO, N. A.; ALECIO, A. C.; KATO, M. J.; YOUNG, C. M.; OLIVEIRA, J. E.; FURLAN, M. Composition and screening of antifungal activity against Cladosporium sphaerospermum and Cladosporium cladosporioides of essential oils of leaves and fruits of Piper species. African Journal of Biotechnology, v. 9, n. 37, p. 6135-6139, 2010. DOI: 10.5897/AJB09.1956.
MORCIA, C.; MALNATI, M.; TERZI, V. In vitro antifungal activity of terpinen-4-ol, eugenol, carvone, 1, 8-cineole (eucalyptol) and thymol against mycotoxigenic plant pathogens. Food Additives & Contaminants: Part A, v. 29, n. 3, p. 415-422, 2012. DOI: 10.1080/19440049.2011.643458.
NIST/EPA/NIH mass spectral library and search/ analysis programs. Hoboken, NJ: John Wiley and Sons, 2010.
PÁNEK, M.; REINPRECHT, L.; HULLA, M. Ten Essential Oils for Beech Wood Protection - Efficacy Against Wood-destroying Fungi and Moulds, and Effect on Wood Discoloration. BioResources, v. 9, n. 3, p. 5588-5603, 2014.
PINHEIRO, C. G.; BIANCHINI, N. H.; BATISTA, B. F.; PAVLACK, A. S.; MUNIZ, M. F. B., PAVANATO, M. A.; GOUVEIA, F. N.; REINIGER, L. R. S.; HEINZMANN, B. M. Hesperozygis ringens (Benth.) Epling essential oil: antifungal activity and effect on ergosterol content of wood-decay fungi. Journal of Essential Oil Research, v. 33, n. 3, p. 265-275, 2021. https://doi.org/10.1080/10412905.2020.1840450.
RASBAND, W. S. ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, https://imagej.nih.gov/ij/.
REINPRECHT, L.; HULLA, M. Colour changes in beech wood modified with essential oils due to fungal and ageing-fungal attacks with Coniophora puteana. Drewno, v. 58, n. 194, p. 37-48, 2015. DOI: 10.12841/wood.1644-3985.114.03.
SCHINDLER, B.; HEINZMANN, B. M. Piper gaudichaudianum Kunth: seasonal characterization of the essential oil chemical composition of leaves and reproductive organs. Brazilian Archives of Biology and Technology, v. 60, e17160441 2017. http://dx.doi.org/10.1590/1678-4324-2017160441.
SIDDIQUE, S.; PERVEEN, Z.; NAWAZ, S.; SHAHZAD, K.; ALI, Z. Chemical composition and antimicrobial activities of essential oils of six species from family Myrtaceae. Journal of Essential Oil Bearing Plants, v. 18, n. 4, p. 950-956, 2015. https://doi.org/10.1080/0972060X.2014.935020.
SILVA, D. T.; BIANCHINI, H. H.; MUNIZ, M. F. B.; HEINZMANN, B. M.; JABIDI, J. Chemical composition and inhibitory effects of Nectandra grandiflora leaves essential oil against wood decay fungi. Drewno, v. 59, n. 197, 2016. http://dx.doi.org/10.12841/wood.1644-3985.C29.10.
SILVA, J. K. R.; ANDRADE, E. H. A.; GUIMARAES, E. F.; MAIA, J. G. S. R. Essential oil composition, antioxidant capacity and antifungal activity of Piper divaricatum. Natural Product Communications, v. 5, n. 3, p. 477-480, 2010. https://doi.org/10.1177/1934578X1000500327.
SILVA, J. K. R. SILVA, N. N.; SANTANA, J. F. S.; ANDRADE, E. H. A.; MAIA, J. G. S.; SETZER, W. N. Phenylpropanoid-rich essential oils of Piper species from the Amazon and their antifungal and anti-cholinesterase activities. Natural Product Communications, v. 11, n. 12, p. 1907-1911, 2016. DOI: https://doi.org/10.1177/1934578X1601101233.
SOIDROU, S. H.; FARAH, A.; SATRANI, B.; GHANMI, M.; JENNAN, S.; HASSANE, S. O. S.; LACHKAR, M.; EL ABED, S.; IBNSOUDA KORAICHI, S.; BOUSTA, D. Fungicidal activity of four essential oils from Piper capense, Piper borbonense and Vetiveria zizanoides growing in Comoros against fungi decay wood. Journal of Essential Oil Research, v. 25, n. 3, p. 216-223, 2013. https://doi.org/10.1080/10412905.2013.767758.
STANGERLIN, D. M.; COSTA, A. F. da.; GONÇALEZ, J. C.; PASTORE, T. C. M.; GARLET, A. Resistência natural da madeira de três espécies amazônicas submetidas ao ataque de fungos apodrecedores. Revista Ciência da Madeira, v. 4, n. 1, p. 15–32, 2013. http://dx.doi.org/10.1590/S0044-59672013000400004.
TOHIDI, B.; RAHIMMALEK, M.; TRINDADE, H. Review on essential oil, extracts composition, molecular and phytochemical properties of Thymus species in Iran. Industrial Crops and Products, v. 134, p. 89–99, 2019. https://doi.org/10.1016/j.indcrop.2019.02.038.
TRES, J. Rendimento e composição química do óleo essencial de raízes de Piper gaudichaudianum Kunth e atividade fungitóxica in vitro. 2017. 85p. Dissertação (Mestrado em Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2017.
VARSHNEY, V. K.; PANDEY, A.; ONIAL, P. K.; DAYAL, R. Antifungal activity of phytochemicals from Eucalyptus hybrid leaves against some plant pathogenic and wood decay fungi. Archives of Phytopathology and Plant Protection, v. 45, n. 19, p. 2347-2354, 2012. https://doi.org/10.1080/03235408.2012.727073.
VICIOLLE, E.; CASTILHO, P.; ROSADO, C. In vitro and in vivo assessment of the effect of Laurus novocanariensis oil and essential oil in human skin. International Journal of Cosmetic Science, v. 34, n. 6, p. 546–550, 2012. https://doi.org/10.1111/j.1468-2494.2012.00745.x.
WU, C. L.; CHIEN, S. C.; WANG, S. Y.; KUO, Y. H.; CHANG, S. T. Structure-activity relationships of cadinane-type sesquiterpene derivatives against wood-decay fungi. Holzforschung, v. 59, n. 6, p. 620-627, 2005. https://doi.org/10.1515/HF.2005.100.
XIE, Y.; WANG, Z.; HUANG, Q.; ZHANG, D. Antifungal activity of several essential oils and major components against wood-rot fungi. Industrial Crops and Products, v. 108, p. 278–285, 2017. https://doi.org/10.1016/j.indcrop.2017.06.041.
ZHANG, Z.; YANG, T.; MI, N.; WANG, Y.; LI, G.; WANG, L.; XIE, Y. Antifungal activity of monoterpenes against wood white-rot fungi. International Biodeterioration & Biodegradation, v. 106, p. 157-160, 2016. https://doi.org/10.1016/j.ibiod.2015.10.018.
Downloads
Publicado
Como Citar
Edição
Seção
Licença
Copyright (c) 2022 Nativa
Este trabalho está licenciado sob uma licença Creative Commons Attribution-NonCommercial 4.0 International License.
Direitos Autorais para artigos publicados nesta revista são do autor, com direitos de primeira publicação para a revista. Em virtude de a aparecerem nesta revista de acesso público, os artigos são de uso gratuito, com atribuições próprias, em aplicações educacionais e não-comerciais.
A artigos publicados nessa revista, podem ser reproduzidos parcialmente ou utilizados como referência por outros autores, desde que seja cita a fonte, ou seja, a Revista Nativa.
Copyright for articles published in this journal are the authors, with first publication rights granted to the journal. The journal shows open access, and articles are free to use, with proper attribution, in educational and non-commercial.
The articles published in this journal may be reproduced in part or used as a reference by other authors, provided that the source is quoted.
