Genetic diversity of potential mother trees of Myracrodruon urundeuva by ISSR markers

Authors

  • Jocelly de Sousa Lopes jocellyslopes@gmail.com
    Universidade Federal de Mato Grosso
  • Márcia Regina Jantsch da Costa marciajantsch22@gmail.com
    Universidade Federal de Mato Grosso
  • Daniele Aparecida Alvarenga Arriel daniarriel@hotmail.com
    Universidade Federal de Uberlândia

DOI:

10.34062/afs.v7i2.9424

Keywords:

genetic dissimilarity, recovery of degraded areas, genetic conservation

Abstract

Myracrodruon urundeuva is a tree species of the family Anacardiaceae, native from the northeast, southeast and midewest regions of Brazil, locally known as aroeira. Because of its long history of predatory exploitation, this species is has been lately classified as vulnerable to extinction. The present work had as objective to evaluate the genetic diversity of potential mother trees of M. urundeuva using ISSR molecular markers in order to subsidize the collection of seeds with large genetic diversity. The selected trees are located in the forest reserve of Brita Guia, in the municipality of Cuiabá – MT, Brazil. Of the 20 ISSR primers tested, seven were selected to characterize genetic variability. The total amplified fragments were 116, with a percentage of 99.15% polymorphism. The individuals presented Nei's genetic diversity (He) of 0.2706 and Shannon's diversity index (I) of 0.4226. The UPGMA grouping method revealed the existence of two large groups among the individuals, which could be considered to perform seed collection among divergent genotypes.

References

Araújo FS, Pacheco MV, Vieira FA, Ferrari CS, Félix F C, Chagas, K P T. (2016) ISSR molecular markers for the study of the genetic diversity of Mimosa caesalpiniaefolia Benth. Idesia, 34: 47-52

Brasil Ministério do Meio Ambiente, Recursos Hídricos e Amazônia Legal (1997). Manejo da bacia hidrográfica do rio Coxipó-açu para a conservação de seus recursos hídricos. Cuiabá: UFMT, ABEAS/SRH, 113p.

Caixeta, ET, Ferrão LFV, Maciel-Zambolim E (2013) Molecular Markers In: Biotechnology applied to plant breeding. Viçosa. P. 31-68.

Carlini EA, Duarte-Almeida J, Rodrigues E, Tabach R (2010) Antiulcer effect of the pepper trees Schinus terebinthifolius Raddi (aroeira-da-praia) and Myracrodruon urundeuva Allemão, Anacardiaceae (aroeira-do-sertão). Brazilian Journal of Pharmacognosy, 20(2): 140-146. doi.org/10.1590/S0102-695X2010000200001

Carvalho, PER (2003) Brazilian tree species, Embrapa Informação Tecnológica, Colombo-PR. v.1, 177p.

Costa DF, Vieira FA, Fajardo CG, Chagas KPT (2015) Genetic diversity and selection of ISSR primers in a natural mangaba population (Hancornia speciosa Gomes) (Apocynaceae). Revista Brasileira de Fruticultura, 37: 970-976. doi.org/10.1590/0100-2945-246/14

Costa RB, Almeida, EV, De Freitas ACA, Schirmbeck AG, Rodrigues NB, Roa RAR (2011) Hybrid vigour of aroeira parent trees for the seeds germination character. Multitemas, 39: 155-162. doi: 10.20435/multi.v0i39.371

Da Silva BM, Rossi APB, Dardengo JFE, Araújo VAAC, Rossi FS, Oliveira LO, Clarindo WR (2016) Genetic diversity estimated using inter-simple sequence repeat markers in commercial crops of cupuassu tree. Ciência Rural, 46(1): 108-113. doi: 10.1590/0103-8478cr20141634

Dias CFT, Bertini CHCM., Silva APMS, Cavalcanti JJV (2015) Genetic variability in early-cycle erect cowpea analysed with RAPD and ISSR markers. In: Revista Ciência Agronômica, 46(3): 563-572. doi: 10.5935/1806-6690.20150039

Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure from small quantities of fresh leaf tissues. Phytochem Bull, 19:11–15.

Farjado CG, Silva RAR, Chagas KTP, Vieira FA (2018) Genetic and phenotypic association of the carnauba palm tree evaluated by inter-simple sequence repeat and biometric traits. Genethics and Molecular Research, 17(3). doi: 10.4238/gmr18018

Freitas MLM, Aukar APA, Sebben AM, Moraes MLT, Lemos EGM (2005) Genetic variability in intra Myracrodruon urundeuva Fr. All. by AFLP marker. Scientia forestalis, 68: 21-28.

Gaino APSC, Moraes, MLT D, Moreira JP, Cardin LT, Moraes MA, Silva AMD, Freitas MLM, Sebbenn AM (2011) Mating system in Myracrodruon urundeuva (Anarcadiaceae): implications for conservation genetics. Brazilian Journal of Botany, 34(4): 545-551. doi.org/10.1590/S0100-84042011000400008

Kageyama PY, Gandara FB (1993) Population dynamics of tree species: implications for management and conservation. In: Simpósio de Ecossistemas da costa Brasileira, Anais, São Paulo.

Lacerda CMB, Kageyama PY, Ferraz EM (1999) Isoenzymatic diversity in Myracrodruon urundeuva in two anthropic situations in the semi-arid. Scientia forestalis, 55: 89-95.

Lorenzi H (2008) Brazilians trees: Manual for identification and cultivation of native tree plants in Brazil. 5th Edition. São Paulo: Plantarum Institute of Flora Studies Ltda. 384p.

Lorenzoni RM, Soares TCB, Santiago VF, Silva JÁ, Coelho RI (2014) Utilização de marcadores ISSR na avaliação da divergência genética entre acessos de biribazeiro. Revista Brasileira de Fruticultura, 36: 251-257. doi.org/10.1590/S0100-29452014000500029

Manfio CE, Motoike SY, Rezende MDV, Santos CEM, Sato AY (2012) Evaluation of macaúba progenies in the juvenile phase and estimation of genetic parameters and genetic diversity. Pesquisa Florestal Brasileira, 32(69): 63-68. doi 104336/2012.pfb.32.69.63

Nei M (1973) Analysis of gene diversity in sunbdivided populatios. Proceedings of the National Academy of Sciences of the United States of America. Washignton, p. 3321-3323.

Nogueira JCB (2010) Mixed reforestation with native species: Ciliary mat. 1st Edition. São Paulo: Forest Institute. 148p.

Paes JB, Morais VM, Lima CR (2004) Natural resistance of nine Brazilian semiarid woods to fungi under laboratory conditions. Revista Árvore, 28(2):275-282. doi: 10.1590/S0100-67622004000200014

Pires IE, Resende MDV, Silva RL, Resende JRMFR (2011) Genética Florestal. Edition 1th, Viçosa, MG: UFV, 318p.

Ramalho AB, Rossi AAB, Dardengo JFE, Zortea KEM, Thiago AV, Martins KC (2016) Genetic diversity among Bertholletia excelsa genotypes using molecular markers. Floresta, Curitiba, v. 46, n. 2, p. 207-214. doi: 10.5380/rf.v46i2.41970

Rocha VD, Tiago PV, Tiago AV, Pedri E C M, Cardoso, E S, & Rossi, A A B (2017) Genetic diversity among Hymenaea courbaril L. genotypes naturally occurring in the north of Mato Grosso State, Brazil. Genetics and Molecular Research, 16(3): 1-9. doi: 10.4238/gmr16039706

Rossi FS, Rossi AA, Dardengo JFE, Brauwers LR, Silva ML, Sebben AM (2014) Genetic diversity in natural populations of Mauritia flexuosa L. f. (Arecaceae) with the use of ISSR markers. Scientia Forestalis, Piracicaba, 42(104): 631-639.

Rohlf FJ (1992). Numerical taxonomy and multivariate analysis system (version 1.70). New York: Exeter Publis Hers, 470p.

Sebbenn AM (2003) Sample size for ex situ conservation of tree species with mixed breeding system. Revista do Instituto Florestal, 15(2):147-162.

Yeh FC, Yang RC, Boyle TBJ, Ye ZH, Mao JX (1997) POPGENE, The User-friendly Shareware for Population Genetic Analysis. Molecular Biology and Biotechnology Centre. University of Alberta, Edmonton, Alberta, CANADA.

Downloads

Published

2020-06-28