Regeneration of Complete Plantlets from Callus Culture of Azadirachta indica A. Juss using Immature Flower Buds

Authors

  • Ashok Gehlot agbios843@gmail.com
    Forest Genetics and Tree Breeding Division, Arid Forest Research Institute, Jodhpur, P. O. Krishi Upaj Mandi, Basni, New Pali Road, Jodhpur-342005 (Rajasthan) INDIA. http://orcid.org/0000-0003-1866-705X
  • Inder Dev Arya agbios843@gmail.com
  • Sarita Arya agbios843@gmail.com

DOI:

10.34062/afs.v4i1.3978

Keywords:

Azadirachta indica, flower buds, callus culture

Abstract

Plant regeneration via callus culture was achieved using immature flower buds of mature tree of Azadirachta indica A. Juss on semi-solid MS medium enriched with 9.05µM 2,4-D + additives. The regenerated callus were amplified on lower concentration of  4.52 µM  2,4-D + additives for micro-shoot regeneration study, the best micro-shoot regenerated from callus was achieved on MS medium supplemented with 8.88  µM  BAP with 3.78±0.50  numbers of shoot and 3.66±0.57 shoot length. The micro-shoots were amplified on 8.88 µM BAP+ 0.27 µM NAA with 5.22±0.53 numbers of shoot and 4.62±0.59 shoot length respectively. Micro shoot were successfully rooted on MS medium supplemented with IBA 4.92 µM with rooting percent (100%), 4.35±0.22 root number and 3.95±0.23 root length. The callus derived plantlets were hardened in vitro and ex vitro and acclimatized in poly house followed by agro-shade house. The efficiency of the protocol will be helpful for propagation of Azadirachta indica plants on a mass scale.

Author Biography

Ashok Gehlot, Forest Genetics and Tree Breeding Division, Arid Forest Research Institute, Jodhpur, P. O. Krishi Upaj Mandi, Basni, New Pali Road, Jodhpur-342005 (Rajasthan) INDIA.

Research Scholar and Forest Genetics and Tree Breeding Division

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Published

2017-03-31