A micropropagation protocol for mass multiplication of Terminalia arjuna - a valuable medicinal tree

Authors

  • Meena Choudhary meenaseervi@gmail.com
    Arid Forest Research Institute, Jodhpur
  • Shipra Jaiswal shipra.afri@gmail.com
    Arid Forest Research Institute, Jodhpur
  • Rakhi Singh rakhisingh_14@yahoo.com
    Arid Forest Research Institute, Jodhpur
  • Inder Dev Arya aryaid@icfre.org
    Arid Forest Research Institute, Jodhpur
  • Sarita Arya aryas@icfre.org
    Arid Forest Research Institute, Jodhpur

DOI:

10.34062/afs.v2i1.2107

Keywords:

Micropropagation technique, Axillary bud culture, In vitro culture.

Abstract

A reliable and efficient micropropagation protocol was developed through axillary shoot proliferation from nodal explants of mature Terminalia arjuna. Season of explants collection and maturity of explants showed direct influence on bud-break. Nodal stem segments collected during the months of April and May gave best response. Nodal segments of fresh sprouts originated from lopped tree of T. arjuna were used as explants for establishment of in vitro culture. Surface sterilized explants produced optimum number of shoots through activation of axillary buds - on modified Murashige and Skoog’s (M-MS) medium. Maximum (100%) in vitro shoot proliferation was obtained on M-MS medium supplemented with 8.86 µM BAP + additives (100 mg L-1 of ascorbic acid, 50 mg L-1 of citric acid, 50 mg L-1 of adenine sulphate and 25 mg L-1 PVP). Modified M-MS medium supplemented with 4.44 µM BAP + 0.54 µM NAA + additives was found to be best for 11.38±0.26 shoot multiplication. After four week of culturing the in vitro regenerated shoots were rooted when pulse treated with 984 µM IBA for 10 min and transferred on hormone free half strength MS medium containing 100 mg L-1 activated charcoal. In vitro regenerated plants were transferred to field after gradual hardening and acclimatization procedure. Present method can be used for large scale commercial production of this medicinally important tree.

Author Biographies

Meena Choudhary, Arid Forest Research Institute, Jodhpur

Forest Genetics Tree Breeding division,

Research scholar

Shipra Jaiswal, Arid Forest Research Institute, Jodhpur

Forest Genetics and Tree Breeding division,

Research Scholar

Rakhi Singh, Arid Forest Research Institute, Jodhpur

Forest Genetics and Tree Breeding division,

Research Scholar

Inder Dev Arya, Arid Forest Research Institute, Jodhpur

Forest Genetics and Tree Breeding division,

Scientist-G

Sarita Arya, Arid Forest Research Institute, Jodhpur

Forest Genetics and Tree Breeding division,

Scientist-F

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Published

2015-03-29