Organogenesis in zygotic embryos and hypocotyls of physic nut (Jatropha curcas L.) under 6-benzylaminopurine levels

Authors

  • Jefferson da Luz Costa advancesfor@gmail.com
    Universidade Federal de Mato Grosso http://www.ufmt.br
  • André Luís Lopes da Silva advancesfor@gmail.com
  • Sidnei Emilio Bordignon Júnior advancesfor@gmail.com
  • Aloísio Freitas Chagas Júnior advancesfor@gmail.com
  • Gessiel Newton Scheidt advancesfor@gmail.com
  • Ronaldo Valadares Veras Júnior advancesfor@gmail.com

DOI:

10.34062/afs.v1i2.1738

Keywords:

Callogenesis, BAP, Tissue culture, In vitro morphogenesis.

Abstract

Physic nut had a great potential for biodiesel production and presents higher oil content. The aim of this study was obtain organogenesis in zygotic embryos and hypocotyls of physic nut using 6-benzylaminopurine (BAP). Seeds were disinfected and after then cotyledons were removed; they were in vitro germinated at 0, 1, 2, 3, 4, 5 and 6 mg.L-1 BAP. Hypocotyls were excised from germinated seeds with 45 days old and cultured at 0, 1, 2.5 and 5 mg.L-1 BAP. Callus from zygotic embryos were cultured at double-phase medium being liquid medium supplemented with 1, 2.5 and 5 mg.L-1 BAP. The largest percentage of callus formation in zygotic embryos occurred at 6 mg.L-1 BAP. On the other hand, to induce shoot from zygotic embryos the best BAP level was 4 mg.L-1. Callogenesis in hypocotyls can be efficiently induced with 1 mg.L-1 BAP. Double-phase medium results in lowest protuberances rate, varying from 0 to 3 per callus. In order to induce callus from zygotic embryos and hypocotyls of physic nut, BAP is sustainable.

Author Biography

Jefferson da Luz Costa, Universidade Federal de Mato Grosso

Professor do Programa de Pós-Graduação em Ciências Florestais e Ambientais, Faculdade de Engenharia Florestal, Universidade Federal de Mato Grosso.

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Published

2014-06-29

Issue

Section

Short Communication