Selection of Eucalyptus urophylla cell lineages for tolerance to hydric and thermal stress

Authors

  • Juliana de Oliveira Fernandes Viana leandroengflor@gmail.com
  • Leandro Silva Oliveira leandroengflor@gmail.com
    Universidade Federal do Mato Grosso, Faculdade de Engenharia Florestal.
  • Priscila de Oliveira Antonelli leandroengflor@gmail.com
  • Katherine Derlene Batagin-Piotto leandroengflor@gmail.com
  • Gilvano Ebling Brondani gebrondani@yahoo.com.br
  • Antônio Natal Gonçalves leandroengflor@gmail.com
  • Marcílio de Almeida mdalmeida@usp.br

DOI:

10.34062/afs.v1i4.1779

Keywords:

In vitro selection, Micropropagation, Cross-tolerance.

Abstract

The present study was aimed at the evaluation of the possible relationship between high temperature sand increased tolerance to water deficiency of Eucalyptus urophylla cell lineages on their physiological and biochemical aspects. For this purpose, calluses cultures derived from hypocotyls Eucalyptus urophylla were maintained in tissue culture in an N7 culture medium with 5 mg L-1 Picloram and used to conduct tests. The calluses were treated at different temperatures (25, 30, 35, 40 and 45°C) for 2 h in BOD and then subjected to a selection pressure in N7 medium supplemented with 20% polyethylene glycol (PEG 6000) for 20 days under constant light exposure (42 μmol m-2 s-1 PAR) and temperature (26±2°C) conditions with a 12 h photoperiod. For evaluate the experiment, proline, total soluble proteins, glucose, polyose and glucuronic acid contents were determined as well as the relative growth rate by measuring dry weight and fresh weight. The results indicated that previous treatment with higher temperatures may increase tolerance to drought stress. Therefore tolerant cell lines to stress conditions can be selected using this methodology.

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Published

2015-01-09